Automated assay for the quantification of dissolved proteins in natural seawater samples
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چکیده
An assay is described that permits the simultaneous determination of concentrations of total dissolved proteins and total "dissolved free amino acids" in natural seawater samples. It can be performed automatically even during field experiments using an AutoAnalyzer system or a modified amino acid analyzer. Except filtration, no further pretreatment of the samples is necessary. The determination is based on the reaction of o-phthaldialdehyde (OPA) with proteins or amino acids, respectively, after separating both components by a column chromatography step. For routine analysis of protein concentrations in natural seawater samples, the lower detection limit is at least 0.5 ngfsample. As the sample volume can be varied between 25 Jll and 2 000 J.Ll, concentrations below 0.5 J.Lg/1 can be analyzed. The determination of protein concentration in a single seawater sample takes about 30 minutes.
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